Z719102
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Z719102
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Basic Methods in Microscopy
| ISBN-10: | 0-87969-751-2 |
| ISBN-13: | 978-0-87969-751-8 |
Description
| General description | Imaging has become a vital tool for researchers in all aspects of biology. Recent advances in microscope technology, labeling techniques and gene and protein manipulation methods have led to breakthroughs in our understanding of biological processes. In order to take advantage of these techniques, biologists need to understand the fundamental techniques of microscopy. The methods found here, drawn from the popular laboratory standard manuals Cells: A Laboratory Manual and Live Cell Imaging provide a solid course in the basics of using the microscope in a biology laboratory. This book provides an essential guide to light microscopy, fluorescence microscopy, confocal microscopy, multiphoton microscopy and electron microscopy, preparation of tissues and cells, labeling of specimens and analysis of cellular events. |
Properties
| publication info | D. Spector and R. Goldman, Cold Spring Harbor Laboratory Press, 2005, 375 pp., soft cover |
Table Of Contents
| Table of Contents | 1. Light Microscopy 2. Confocal Microscopy, Deconvolution, and Structured Illumination Methods 3. Multiphoton and Multispectral Laser-scanning Microscopy 4. Preparation of Cells and Tissues for Fluorescence Microscopy 5. Nonimmunological Fluorescent Labeling of Cellular Structures 6. Introduction to Immunofluorescence Microscopy 7. Immunostaining of Microtubules, Microtubule-associated Proteins, and Intermediate Filaments 8. Immunofluorescence Localization of Actin 9. Immunofluorescence Localization of Nuclear Proteins 10. Immunofluorescence Methods for Saccharomyces cerevisiae 11. Immunofluorescence Methods for Drosophila Tissues 12. Immunofluorescence Methods for Caenorhabditis elegans 13. Analyzing DNA Replication: Nonisotopic Labeling 14. Analyzing RNA Synthesis: Nonisotopic Labeling 15. Fluorescence In Situ Hybridization to DNA 16. Whole Mount Fluorescence In Situ Hybridization to Drosophila Chromosomal DNA 17. In Situ Hybridization to RNA 18. Whole Mount In Situ Detection of RNAs in Vertebrate Embryos and Isolated Organs 19. Image Production Using Transmission Electron Microscopy 20. Preparative Methods for Transmission Electron Microscopy 21. Immunoelectron Microscopy APPENDIX 1. Microscopy: Lenses, Filters, and Emission/Excitation Spectra APPENDIX 2. Localization Markers for Subcellular Components |
